7, 12-Dimethylbenz(a)anthracene retention in the rat submandibular gland following intraglandular injection.

6 hr to 6 weeks by liquid scintillation spectroscopy. Ethyl acetate extracts of gland homogenates and cell fraction sus pensions were similarly analyzed to determine the degree of binding of the carcinogen in this tissue. Less than 30% of the total hydrocarbon injected was found in the glands of animals sacrificed during the 1st week. The carcinogen content of the gland declined to 13, 7, and 5%, respectively at 2, 4, and 6 weeks after treatment. A minimum of 65% of the radioactivity in this tissue was extractable from the homogenates of all the groups examined. The nuclear frac tion contained higher values of 14C than other fractions